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antibodies against cell surface markers expressed on mapc-derived ecs  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology antibodies against cell surface markers expressed on mapc-derived ecs
    Antibodies Against Cell Surface Markers Expressed On Mapc Derived Ecs, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+surface+marker+expression/antibodies+against+cell+surface+markers+expressed+on+mapc+derived+ecs/us08192348-415-9-24
    Average 90 stars, based on 1 article reviews
    antibodies against cell surface markers expressed on mapc-derived ecs - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: CD74 is a functional MIF receptor on activated CD4 + T cells
    Article Snippet: For detection of CD74/CXCR4 protein complexes, 72 h-activated CD4 + T cells were stimulated with MIF in indicated concentrations for 40 min following fixation and PLA using the DuolinkTM InSitu Orange Starter Kit Mouse/Rabbit (DUO92102) from Sigma Aldrich. .. For immunofluorescent staining and PLA, the Duolink ® PLA fluorescence protocol provided by the manufacturer was essentially followed, using primary antibodies against CD74 (sc-6262, 1:100, Santa Cruz) and CXCR4 (PA3-305, 1:800, ThermoFisher) as described above. .. Samples were then prepared for microscopy using Duolink ® mounting medium with DAPI, and coverslips sealed with commercially available nail polish and stored at − 20 °C until imaging on a Zeiss LSM880 AiryScan confocal microscope was performed.

    Article Title: L1CAM signaling through planar cell polarity generates SOX2 + metastatic progenitors in lung adenocarcinoma
    Article Snippet: Additional hematoxylin and eosin (H&E) staining was performed by Histowiz, Inc (Brooklyn, NY, US). .. For immunofluorescence staining, samples were fixed in 4% PFA for 10 min and permeabilized with 0.5% of Triton X-100 in PBS for another 10 min. After incubating with 10% normal goat serum (Life Technologies Cat# 50062Z) for 1 h at room temperature, the samples were incubated with primary antibodies overnight at 4°C in blocking solution with antibodies against mouse L1CAM (Miltenyi Biotec Cat# 130-115-812, AB_2727206), human L1CAM (Santa Cruz Biotechnology Cat# sc-53386, RRID: AB_628937), Sox2 (Invitrogen Cat# 14-9811-82, RRID: AB_891383), CELSR1 (Millipore Sigma Cat# ABT119, RRID: AB_11215810), c-Jun(pS73) (Cell Signaling Technology Cat# 9164, RRID: AB_330892), GFP (Aves Labs Cat# GFP-1010, RRID: AB_2307313), mouse FZD6 (R&D Systems Cat# AF1526, RRID: AB_354842), human FZD6 (Abcam Cat# AB150545, RRID: AB_3697520), Sox9 (Invitrogen Cat# 14-9765-82, RRID:AB_2573006), Cleaved Caspase-3 (Cell Signaling Technology Cat# 9661, RRID: AB_2341188), anti-TTF1/NKX2-1 (abcam Cat# ab76013, RRID: AB_1310784), or human cytokeratin (Dako Cat# M3515, RRID: AB_2132885). .. After washing in PBS three times, samples were incubated with fluorophore-conjugated secondary antibodies for 1 h at room temperature in the dark, followed by another round of PBS washes and staining of nuclei with Hoechst 33342 (Thermo Fisher Scientific Cat# H3570).

    Proximity Ligation Assay:

    Article Title: CD74 is a functional MIF receptor on activated CD4 + T cells
    Article Snippet: For detection of CD74/CXCR4 protein complexes, 72 h-activated CD4 + T cells were stimulated with MIF in indicated concentrations for 40 min following fixation and PLA using the DuolinkTM InSitu Orange Starter Kit Mouse/Rabbit (DUO92102) from Sigma Aldrich. .. For immunofluorescent staining and PLA, the Duolink ® PLA fluorescence protocol provided by the manufacturer was essentially followed, using primary antibodies against CD74 (sc-6262, 1:100, Santa Cruz) and CXCR4 (PA3-305, 1:800, ThermoFisher) as described above. .. Samples were then prepared for microscopy using Duolink ® mounting medium with DAPI, and coverslips sealed with commercially available nail polish and stored at − 20 °C until imaging on a Zeiss LSM880 AiryScan confocal microscope was performed.

    Fluorescence:

    Article Title: CD74 is a functional MIF receptor on activated CD4 + T cells
    Article Snippet: For detection of CD74/CXCR4 protein complexes, 72 h-activated CD4 + T cells were stimulated with MIF in indicated concentrations for 40 min following fixation and PLA using the DuolinkTM InSitu Orange Starter Kit Mouse/Rabbit (DUO92102) from Sigma Aldrich. .. For immunofluorescent staining and PLA, the Duolink ® PLA fluorescence protocol provided by the manufacturer was essentially followed, using primary antibodies against CD74 (sc-6262, 1:100, Santa Cruz) and CXCR4 (PA3-305, 1:800, ThermoFisher) as described above. .. Samples were then prepared for microscopy using Duolink ® mounting medium with DAPI, and coverslips sealed with commercially available nail polish and stored at − 20 °C until imaging on a Zeiss LSM880 AiryScan confocal microscope was performed.

    Nucleic Acid Electrophoresis:

    Article Title: Tranilast inhibits the expression of genes related to epithelial-mesenchymal transition and angiogenesis in neurofibromin-deficient cells
    Article Snippet: .. Equal amounts of total lysates were fractionated by SDS-polyacrylamide gel electrophoresis, and the separated proteins were transferred to a nitrocellulose filter and probed with primary antibodies to fibronectin (Santa Cruz Biotechnology), to N-cadherin (Santa Cruz Biotechnology), to collagen type I (Abcam), or to α-tubulin (Sigma-Aldrich). .. Immune complexes were detected with horseradish peroxidase–conjugated secondary antibodies (GE Healthcare and Dako), enhanced chemiluminescence reagents (ImmunoStar LD, Wako), and an LAS-3000mini instrument (GE Healthcare).

    Western Blot:

    Article Title: CD8 T cell response and its released cytokine IFN-γ are necessary for lung alveolar epithelial repair during bacterial pneumonia
    Article Snippet: Anti-human ProSPC (Novus Biologicals, NBP1-60117) and anti-human Ep-CAM (Santa Cruz Biotechnology, 25308) were used for human lung studies. .. The following antibodies were used for western blotting: mouse monoclonal (E-1) anti-homeodomain only protein HOPX (1:1000, Santa Cruz, sc-398703), mouse monoclonal (C4) anti-gizzard Actin of chicken origin β-Actin (1:1000, Santa Cruz, sc-47778), human anti-RAGE (R&D Systems AF1145) and β-Actin (Sigma A5441). .. EdU incorporation assay was performed using Click-iT® EdU (5-ethynyl-2’-deoxyuridine) Alexa Fluor® Imaging Kit (ThermoFisher Scientific).

    Article Title: MiR-661 promotes tumor invasion and metastasis by directly inhibiting RB1 in non small cell lung cancer
    Article Snippet: Firefly and renilla luciferase activities were measured at 48 h after cotransfection with the Dual-Luciferase Reporter Assay kit (Promega, Madison, Wis, USA). .. Protein expression was assessed by immunoblot analysis of cell lysates (20–60 μg) in RIPA buffer in the presence of rabbit antibodies to E-cadherin, mouse antibodies to β-catenin, fibronectin, vimentin, β-actin (1:500; Santa Cruz, California, USA); and rabbit antibodies to RB1, GAPDH, E2F1, (1:1000; CST, Danvers, MA). .. Quantifications were carried out using Scion Image software and normalized to β-actin levels.

    Expressing:

    Article Title: MiR-661 promotes tumor invasion and metastasis by directly inhibiting RB1 in non small cell lung cancer
    Article Snippet: Firefly and renilla luciferase activities were measured at 48 h after cotransfection with the Dual-Luciferase Reporter Assay kit (Promega, Madison, Wis, USA). .. Protein expression was assessed by immunoblot analysis of cell lysates (20–60 μg) in RIPA buffer in the presence of rabbit antibodies to E-cadherin, mouse antibodies to β-catenin, fibronectin, vimentin, β-actin (1:500; Santa Cruz, California, USA); and rabbit antibodies to RB1, GAPDH, E2F1, (1:1000; CST, Danvers, MA). .. Quantifications were carried out using Scion Image software and normalized to β-actin levels.

    Article Title: Increased synthesis and deposition of extracellular matrix proteins leads to endoplasmic reticulum stress in the trabecular meshwork
    Article Snippet: .. The effect of fibronectin overexpression on ER stress was examined by transducing GTM3 cells with lentiviral fibronectin expression particles (SC-400082-LAC, Santa Cruz Biotechnology, Dallas, TX) for 2 days according the manufacturer instructions. ..

    other:

    Article Title: Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum
    Article Snippet: The goat polyclonal anti-GABA A α1 subunit antibody (A-20; catalog #: SC-31405) and mouse monoclonal anti-nAChR α4 subunit ( CHRNA4 ) antibody (catalog #: sc-74519) were obtained from Santa Cruz Biotechnology (Dallas, TX).

    Immunofluorescence:

    Article Title: L1CAM signaling through planar cell polarity generates SOX2 + metastatic progenitors in lung adenocarcinoma
    Article Snippet: Additional hematoxylin and eosin (H&E) staining was performed by Histowiz, Inc (Brooklyn, NY, US). .. For immunofluorescence staining, samples were fixed in 4% PFA for 10 min and permeabilized with 0.5% of Triton X-100 in PBS for another 10 min. After incubating with 10% normal goat serum (Life Technologies Cat# 50062Z) for 1 h at room temperature, the samples were incubated with primary antibodies overnight at 4°C in blocking solution with antibodies against mouse L1CAM (Miltenyi Biotec Cat# 130-115-812, AB_2727206), human L1CAM (Santa Cruz Biotechnology Cat# sc-53386, RRID: AB_628937), Sox2 (Invitrogen Cat# 14-9811-82, RRID: AB_891383), CELSR1 (Millipore Sigma Cat# ABT119, RRID: AB_11215810), c-Jun(pS73) (Cell Signaling Technology Cat# 9164, RRID: AB_330892), GFP (Aves Labs Cat# GFP-1010, RRID: AB_2307313), mouse FZD6 (R&D Systems Cat# AF1526, RRID: AB_354842), human FZD6 (Abcam Cat# AB150545, RRID: AB_3697520), Sox9 (Invitrogen Cat# 14-9765-82, RRID:AB_2573006), Cleaved Caspase-3 (Cell Signaling Technology Cat# 9661, RRID: AB_2341188), anti-TTF1/NKX2-1 (abcam Cat# ab76013, RRID: AB_1310784), or human cytokeratin (Dako Cat# M3515, RRID: AB_2132885). .. After washing in PBS three times, samples were incubated with fluorophore-conjugated secondary antibodies for 1 h at room temperature in the dark, followed by another round of PBS washes and staining of nuclei with Hoechst 33342 (Thermo Fisher Scientific Cat# H3570).

    Incubation:

    Article Title: L1CAM signaling through planar cell polarity generates SOX2 + metastatic progenitors in lung adenocarcinoma
    Article Snippet: Additional hematoxylin and eosin (H&E) staining was performed by Histowiz, Inc (Brooklyn, NY, US). .. For immunofluorescence staining, samples were fixed in 4% PFA for 10 min and permeabilized with 0.5% of Triton X-100 in PBS for another 10 min. After incubating with 10% normal goat serum (Life Technologies Cat# 50062Z) for 1 h at room temperature, the samples were incubated with primary antibodies overnight at 4°C in blocking solution with antibodies against mouse L1CAM (Miltenyi Biotec Cat# 130-115-812, AB_2727206), human L1CAM (Santa Cruz Biotechnology Cat# sc-53386, RRID: AB_628937), Sox2 (Invitrogen Cat# 14-9811-82, RRID: AB_891383), CELSR1 (Millipore Sigma Cat# ABT119, RRID: AB_11215810), c-Jun(pS73) (Cell Signaling Technology Cat# 9164, RRID: AB_330892), GFP (Aves Labs Cat# GFP-1010, RRID: AB_2307313), mouse FZD6 (R&D Systems Cat# AF1526, RRID: AB_354842), human FZD6 (Abcam Cat# AB150545, RRID: AB_3697520), Sox9 (Invitrogen Cat# 14-9765-82, RRID:AB_2573006), Cleaved Caspase-3 (Cell Signaling Technology Cat# 9661, RRID: AB_2341188), anti-TTF1/NKX2-1 (abcam Cat# ab76013, RRID: AB_1310784), or human cytokeratin (Dako Cat# M3515, RRID: AB_2132885). .. After washing in PBS three times, samples were incubated with fluorophore-conjugated secondary antibodies for 1 h at room temperature in the dark, followed by another round of PBS washes and staining of nuclei with Hoechst 33342 (Thermo Fisher Scientific Cat# H3570).

    Blocking Assay:

    Article Title: L1CAM signaling through planar cell polarity generates SOX2 + metastatic progenitors in lung adenocarcinoma
    Article Snippet: Additional hematoxylin and eosin (H&E) staining was performed by Histowiz, Inc (Brooklyn, NY, US). .. For immunofluorescence staining, samples were fixed in 4% PFA for 10 min and permeabilized with 0.5% of Triton X-100 in PBS for another 10 min. After incubating with 10% normal goat serum (Life Technologies Cat# 50062Z) for 1 h at room temperature, the samples were incubated with primary antibodies overnight at 4°C in blocking solution with antibodies against mouse L1CAM (Miltenyi Biotec Cat# 130-115-812, AB_2727206), human L1CAM (Santa Cruz Biotechnology Cat# sc-53386, RRID: AB_628937), Sox2 (Invitrogen Cat# 14-9811-82, RRID: AB_891383), CELSR1 (Millipore Sigma Cat# ABT119, RRID: AB_11215810), c-Jun(pS73) (Cell Signaling Technology Cat# 9164, RRID: AB_330892), GFP (Aves Labs Cat# GFP-1010, RRID: AB_2307313), mouse FZD6 (R&D Systems Cat# AF1526, RRID: AB_354842), human FZD6 (Abcam Cat# AB150545, RRID: AB_3697520), Sox9 (Invitrogen Cat# 14-9765-82, RRID:AB_2573006), Cleaved Caspase-3 (Cell Signaling Technology Cat# 9661, RRID: AB_2341188), anti-TTF1/NKX2-1 (abcam Cat# ab76013, RRID: AB_1310784), or human cytokeratin (Dako Cat# M3515, RRID: AB_2132885). .. After washing in PBS three times, samples were incubated with fluorophore-conjugated secondary antibodies for 1 h at room temperature in the dark, followed by another round of PBS washes and staining of nuclei with Hoechst 33342 (Thermo Fisher Scientific Cat# H3570).

    Over Expression:

    Article Title: Increased synthesis and deposition of extracellular matrix proteins leads to endoplasmic reticulum stress in the trabecular meshwork
    Article Snippet: .. The effect of fibronectin overexpression on ER stress was examined by transducing GTM3 cells with lentiviral fibronectin expression particles (SC-400082-LAC, Santa Cruz Biotechnology, Dallas, TX) for 2 days according the manufacturer instructions. ..



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